Fig. 4
From: In vivo and in vitro efficacy of crocin against Echinococcus multilocularis
a–c Effects of crocin against E. multilocularis protoscoleces. a Viability of protoscoleces cultured in vitro. The protoscoleces were exposed to crocin for 7 days. To evaluate the survival of protoscoleces, a 0.1% eosin staining exclusion method was used daily. The positive control treatment was 15 μM albendazole sulphoxide (ABZSO). The data are presented as the mean ± SD obtained from three independent experiments. b Effects of crocin treatments on morphology and structural integrity. After treatment with crocin for 5 days, eosin staining was used to observe the protoscoleces. Scale bar = 200 μM. c Scanning electron microscopy analysis of E. multilocularis protoscoleces exposed to 10 μM crocin for 5 days. Scale bar = 20 μM