Fig. 1

OSCP is expressed in the ookinete and oocyst stages. a Schematic representation of the OSCP amino acid sequence. Polyclonal antibodies were prepared against peptide-comprising amino acids 926 to 939 (anti-OSCP α-1); a mixture of peptides and amino acids 1,789–1,802 plus 3,103–3,116 (anti-OSCP α-2) antibodies is shown. b Immunofluorescence analysis of OSCP expression by ookinetes. OSCP localized to the ookinete surface. The ookinete parasite was labeled twice with antibodies against GFP (green: center panel) and OSCP (red: right panel), stained with the DAPI nuclear stain (blue), and showing differential interference contrast (DIC: left panel), as indicated. Negative control was performed as only anti-GFP antibody incubation on unfixed (live) parasites (w/o Anti-OSCP). Scale-bars: 5 µm. c OSCP is localized to the oocyst capsule. Images of an oocyst 15 days after the blood meal