OSCP is involved in ookinete locomotion. a WT and KO ookinete formation in vitro. b Comparison of ookinete dimensions. Green line: length, red line: width. Number of ookinete analyzed: n = 20 (WT) and n = 20 (KO). c Comparison of ookinete gliding speed. Data pooled from 20 measurements for each WT and KO parasites. Welch’s t-test: ***P < 0.001 d Upper panels: Schematic diagram of spiral movement. Each turn around a spiral axis is comprised of two directional changes. Lower panels: examples of trajectories over 10 min. e Comparison of WT and KO ookinete number of turns. Corrected KO is WT ookinete speed/KO ookinete speed (6.74/4.53 = 1.49; panel c) multiplied by number of KO ookinete turns. The number of KO ookinete turns is significantly lower regardless of correction. Welch’s t-test: *P < 0.05 **P < 0.01.