Fig. 1

Blood meals supplemented with glucose are beneficial for DENV replication and transmission in Aedes aegypti. The total RNA of individual whole mosquitoes was collected 3 (a) and 7 (b) days after blood meal consumption. The relative levels of the DENV genome were quantified using real-time PCR analysis. Numbers denotes total number of infected mosquitoes. All examined groups were demonstrated to be statistically significant using ANOVA; *P < 0.05, **P < 0.01. c Salivary proteins were collected from pools of hundreds of mosquitoes at 3, 5, 7, and 9 days after the consumption of infectious blood meals with or without additional supplementation of 0.1% and 1% glucose. E protein expression was determined via western blotting. Purified serum from mouse routinely bitten by naïve mosquitos was used to serve as a primary antibody for internal control of total salivary proteins