Fig. 5From: Identification of the Bcl-2 and Bax homologs from Rhipicephalus haemaphysaloides and their function in the degeneration of tick salivary glandsExpression of recombinant RhBcl-2 and RhBax. a, b Purification of recombinant RhBcl-2 and RhBax. Lane 1: M: the protein marker; Lane 2: non-induced; Lane 3: 1Â mM IPTG induced expression for 8Â h; Lane 4: recombinant protein after purification. c, d Western-blotting detection of RhBcl-2 and RhBax. Purified RhBcl-2 and RhBax were immunoblotted with the anti-sera against RhBcl-2 and RhBax, respectivelyBack to article page