Skip to main content
Fig. 7 | Parasites & Vectors

Fig. 7

From: Identification of the Bcl-2 and Bax homologs from Rhipicephalus haemaphysaloides and their function in the degeneration of tick salivary glands

Fig. 7

Biological effects of RNAi of Rhipicephalus haemaphysaloides RhBcl-2 and RhBax. a Images comparing females inoculated with dsRhBcl-2 and RhBax with control groups on day 7 of feeding. DsRhBcl-2 inhibited the blood-feeding process and resulted in females smaller than the controls. Scale bars: 5 mm. b Confirmation of RhBcl-2 and RhBax silencing using RT-qPCR. Total RNA was extracted on feeding day 5 from whole bodies of female ticks injected with dsRNA. Bars represent mean relative expression of RhBcl-2 and RhBax; error bars represent standard error. *P < 0.05, based on two-tailed Student’s t tests. c TUNEL staining assays of apoptosis levels after interference with the salivary glands by dsRNA of RhBcl-2 and RhBax. DNA was stained with DAPI. Scale bars: 50 μm. d Confirmation of RhBcl-2 and RhBax silencing using RT-qPCR. e Tunel mean fluorescence intensity showed the apoptosis levels. *P < 0.05

Back to article page

Parasites & Vectors

ISSN: 1756-3305

Contact us