Aedes aegypti continuously exposed to Bacillus thuringiensis svar. israelensis does not exhibit changes in life traits but displays increased susceptibility for Zika virus

Table 1 Life traits of Aedes aegypti from RecBti and RecL strains

Parameters	RecBti		RecL		Student’s t-test^a
Parameters	n	Mean ± SD	n	Mean ± SD	Student’s t-test^a
Fecundity (mean no. eggs/female)	8988^b	93.2 ± 3.3	8463^b	86.5 ± 7.6	t₍₄₎ = 2.77, P = 0.43
Fertility (% first-instar larvae)	2551	90.6 ± 2.8	2526	89.9 ± 3.7	t₍₄₎ = 2.77, P = 0.79
Pupal weight (mg), per pool of 25 males	16	69.1 ± 3.4	16	67.3 ± 3.9	t₍₄₎ = 2.77, P = 0.15
Pupal weight (mg), per pool of 25 females	13	122.2 ± 7.4	12	121 ± 4.5	t₍₄₎ = 2.77, P = 0.61
Development time (days)^c	864	10.4 ± 0.5	873	11.1 ± 1.1	t₍₄₎ = 2.77, P = 0.09
Adult rate (%)	900	96 ± 2.2	900	97 ± 2.1	t₍₄₎ = 2.77, P = 0.77
Sex ratio (male:female)	864	1.1:0.9 ± 0.1	873	1.1:0.9	t₍₄₎ = 2.77, P = 0.74
Haematophagic rate (% blood-fed)	120	80 ± 12.6	120	89.2 ± 8.0	t₍₄₎ = 2.77, P = 0.17

^aFor P ≥ 0.05, the mean of each parameter was not significantly different according to Student’s t-test
^bNumber of 8988 and 8463 eggs laid by 96 and 108 blood-fed females, respectively
^cPeriod from first-instar larvae to emergence

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