Table 1 Specificity of multiplex assays evaluated with gDNA of adult nematodes, expressed by Cq values
DNA template | Detection system | |||||
|---|---|---|---|---|---|---|
Multiplex1 | Multiplex2 | |||||
Haemonchus | Teladorsagia | Trichostrongylus | Nematodirus | Ashworthius | Chabertia | |
Haemonchus contortus | 23.51 | nd | nd | nd | nd | nd |
Teladorsagia circumcincta | nd | 25.54 | nd | 34.73a | nd | nd |
Trichostrongylus colubriformis | nd | nd | 24.05 | nd | nd | nd |
Nematodirus battus | nd | nd | nd | 26.23 | nd | nd |
Ashworthius sidemi | nd | nd | nd | nd | 27.07 | nd |
Chabertia ovina | nd | nd | nd | nd | nd | 25.18 |
Cooperia curticei | nd | nd | nd | nd | nd | nd |
Cooperia pectinata | nd | nd | nd | nd | nd | nd |
Oesophagostomum columbianum | nd | nd | nd | nd | nd | nd |