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Table 1 Specificity of multiplex assays evaluated with gDNA of adult nematodes, expressed by Cq values

From: The identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays

DNA template Detection system
Multiplex1 Multiplex2
Haemonchus Teladorsagia Trichostrongylus Nematodirus Ashworthius Chabertia
Haemonchus contortus 23.51 nd nd nd nd nd
Teladorsagia circumcincta nd 25.54 nd 34.73a nd nd
Trichostrongylus colubriformis nd nd 24.05 nd nd nd
Nematodirus battus nd nd nd 26.23 nd nd
Ashworthius sidemi nd nd nd nd 27.07 nd
Chabertia ovina nd nd nd nd nd 25.18
Cooperia curticei nd nd nd nd nd nd
Cooperia pectinata nd nd nd nd nd nd
Oesophagostomum columbianum nd nd nd nd nd nd
  1. aCq value that corresponds to nonspecific amplification. Abbreviation: nd, no DNA was amplified