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Table 2 Evaluation of sensitivity and limit of detection of detection systems in multiplex assays expressed by Cq values

From: The identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays

Mixed template DNA

Detection system

Multiplex1

Multiplex2

Haemonchus

Teladorsagia

Trichostrongylus

Nematodirus

Ashworthius

Chabertia

50 ng

16.54

17.29

17.54

18.79

18.82

16.23

5 ng

19.82

20.50

21.05

22.34

22.26

19.80

500 pg

23.70

24.32

24.67

26.31

25.86

23.42

50 pg

27.35

27.96

28.16

30.01

29.48

26.91

5 pg

31.10

32.18

31.60

34.06

34.61

30.11

500 fg

34.74

35.90

35.23

38.41

nd

33.54

50 fg

nd

nd

nd

nd

nd

nd

  1. The sensitivity was assessed with equimolar mixtures of gDNAs of adult nematodes (in particular, DNA template for Multiplex1 consisted of H. contortus, T. circumcincta, and T. colubriformis, and Multiplex2 of N. battus, A. sidemi, and Ch. ovina). Abbreviation: nd, no DNA was detected
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ISSN: 1756-3305

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