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Table 2 Evaluation of sensitivity and limit of detection of detection systems in multiplex assays expressed by Cq values

From: The identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays

Mixed template DNA Detection system
Multiplex1 Multiplex2
Haemonchus Teladorsagia Trichostrongylus Nematodirus Ashworthius Chabertia
50 ng 16.54 17.29 17.54 18.79 18.82 16.23
5 ng 19.82 20.50 21.05 22.34 22.26 19.80
500 pg 23.70 24.32 24.67 26.31 25.86 23.42
50 pg 27.35 27.96 28.16 30.01 29.48 26.91
5 pg 31.10 32.18 31.60 34.06 34.61 30.11
500 fg 34.74 35.90 35.23 38.41 nd 33.54
50 fg nd nd nd nd nd nd
  1. The sensitivity was assessed with equimolar mixtures of gDNAs of adult nematodes (in particular, DNA template for Multiplex1 consisted of H. contortus, T. circumcincta, and T. colubriformis, and Multiplex2 of N. battus, A. sidemi, and Ch. ovina). Abbreviation: nd, no DNA was detected