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Table 3 Repeatability and reproducibility of multiplex real-time PCR reactions

From: The identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays

Plasmid copies per µl Median ± SD a CV (%)
5 × 107 14.49 ± 0.11 0.8
1 × 107 16.74 ± 0.04 0.2
5 × 106 17.78 ± 0.05 0.3
1 × 106 20.02 ± 0.10 0.5
5 × 105 21.12 ± 0.05 0.2
1 × 105 23.30 ± 0.10 0.4
5 × 104 24.44 ± 0.06 0.3
1 × 104 26.84 ± 0.08 0.3
  1. a Median ± standard deviation (SD) and coefficient of variation (CV) of Cq values of three repetitions of H. contortus ITS2 plasmid standard