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Table 4 Functional verification of the multiplex assays based on analysis of 44 faecal samples

From: The identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays

No. of samples tested

No. of samples test-positive by the faecal flotation technique

No. of samples test-positive by multiplex real-time PCR

Total

Strongylid-type eggs

Nematodirus spp.

Total

Haemonchus

Teladorsagia

Trichostrongylus

Chabertia

Mixed infections

44 (100.0%)

40 (90.9%)

40 (90.9%)

6a (13.6%)

43 (97.7%)

39 (88.6%)

36 (81.8%)

27 (61.4%)

32 (72.7%)

43/44 (97.7%)

  1. Each sample was subjected to quantitative coprological examination using the concentration McMaster technique for enumeration of strongylid-type eggs and eggs of Nematodirus spp. (includes all species except N. battus). After DNA extraction, multiplex real-time PCR analysis was performed, targeting within two assays Haemonchus (H. contortus, H. placei), Teladorsagia (T. circumcincta, T. trifurcata), Trichostrongylus (T. colubriformis, T. vitrinus, and T. rugatus), and Chabertia (Ch. ovina). Nematodirus (N. battus) and Ashworthius (A. sidemi) DNA was not detected
  2. a N. battus was described in one sample