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Table 4 Functional verification of the multiplex assays based on analysis of 44 faecal samples

From: The identification and semi-quantitative assessment of gastrointestinal nematodes in faecal samples using multiplex real-time PCR assays

No. of samples tested No. of samples test-positive by the faecal flotation technique No. of samples test-positive by multiplex real-time PCR
Total Strongylid-type eggs Nematodirus spp. Total Haemonchus Teladorsagia Trichostrongylus Chabertia Mixed infections
44 (100.0%) 40 (90.9%) 40 (90.9%) 6a (13.6%) 43 (97.7%) 39 (88.6%) 36 (81.8%) 27 (61.4%) 32 (72.7%) 43/44 (97.7%)
  1. Each sample was subjected to quantitative coprological examination using the concentration McMaster technique for enumeration of strongylid-type eggs and eggs of Nematodirus spp. (includes all species except N. battus). After DNA extraction, multiplex real-time PCR analysis was performed, targeting within two assays Haemonchus (H. contortus, H. placei), Teladorsagia (T. circumcincta, T. trifurcata), Trichostrongylus (T. colubriformis, T. vitrinus, and T. rugatus), and Chabertia (Ch. ovina). Nematodirus (N. battus) and Ashworthius (A. sidemi) DNA was not detected
  2. a N. battus was described in one sample