|
No. of samples tested
|
No. of samples test-positive by the faecal flotation technique
|
No. of samples test-positive by multiplex real-time PCR
|
|---|
|
Total
|
Strongylid-type eggs
|
Nematodirus spp.
|
Total
|
Haemonchus
|
Teladorsagia
|
Trichostrongylus
|
Chabertia
|
Mixed infections
|
|---|
|
44 (100.0%)
|
40 (90.9%)
|
40 (90.9%)
|
6a (13.6%)
|
43 (97.7%)
|
39 (88.6%)
|
36 (81.8%)
|
27 (61.4%)
|
32 (72.7%)
|
43/44 (97.7%)
|
- Each sample was subjected to quantitative coprological examination using the concentration McMaster technique for enumeration of strongylid-type eggs and eggs of Nematodirus spp. (includes all species except N. battus). After DNA extraction, multiplex real-time PCR analysis was performed, targeting within two assays Haemonchus (H. contortus, H. placei), Teladorsagia (T. circumcincta, T. trifurcata), Trichostrongylus (T. colubriformis, T. vitrinus, and T. rugatus), and Chabertia (Ch. ovina). Nematodirus (N. battus) and Ashworthius (A. sidemi) DNA was not detected
- a N. battus was described in one sample
