Table 1 Primers used to obtain partial small subunit rRNA gene sequences of the salmonid x-cell parasite, and primers and probe of the real-time PCR assay with Per primers

18c

TGGTTGATCCTGCCAGT

Outside, 5′ end

PerF2

TTCTGACCTATCAGCTATGGACG

280–302

RosF2

CCATAAACTATGCCGACTAGG

1019–1039

RosR2

ATCCTTCCGCAGGTTCACCTACGG

Outside, 3′ end

RosR3

TAGTCGGCATAGTTTATGGTTAGG

1037–1014

1492R

TACGGYTACCTTGTTACGACTT

Outside, 3′ end

UnR

GACGGTATCTGATCGTCTT

1008–990

PER-F

CCCTGCTAAATAGTATGCGGTATACA

1332–1357

PER-R

ACCTTCAAAATAAGAACAATCAGCAA

1402–1377

PER-probe

6FAM-AGCGTTGAGCGGAT-MGB-NFQ

1373–1360