Skip to main content
Fig. 4 | Parasites & Vectors

Fig. 4

From: A loop-mediated isothermal amplification assay for Schistosoma mansoni detection in Biomphalaria spp. from schistosomiasis-endemic areas in Minas Gerais, Brazil

Fig. 4

Optimization and validation of the loop-mediated isothermal amplification (LAMP) assay. LAMP products were visualized either by silver-stained 6% polyacrylamide gels or visual inspection of reaction tubes, with a 1:10 dilution of SYBR Green I, by the naked eye (positive, yellow-green; negative, orange) or exposure to ultraviolet (UV) light (positive, fluorescent; negative, non-fluorescent). a Specificity analysis of the optimized LAMP assay. No cross-reactivity was detected. b Analytical limit of detection. Optimized LAMP detected up to 10–1 ng of S. mansoni DNA. c Validation of the optimized LAMP assay using snails maintained in the laboratory at different stages of infection and pooled conditions. S. mansoni DNA was detected in all conditions. B− Negative snail, B+ Biomphalaria snail infected with S. mansoni, Nt cercariae belonging to the family Notocotylidae, Dp cercariae belonging to the family Diplostomidae, B1 snail 1 day post-infection (dpi), B7 snail 7 dpi, B14 snail 14 dpi, B28 snail 28 dpi, P1 pool of 19 negative snails plus one snail at the pre-patent period of infection, P2 pool of 19 negative snails plus one snail shedding cercariae; for other abbreviations, see Figs.  2 and 3

Back to article page