Establishment and validation of a guinea pig model for human congenital toxoplasmosis

Parasites & Vectors

Table 2 Overview of the individual litters of guinea pig dams inoculated with Toxoplasma gondii oocysts or serving as controls. Each line represents a single litter

Animal number	Inoculation dose^a	Day of inoculation^b	Duration of trial^c	Litter size^d	Course of pregnancy	Serology mother^e	qPCR mother^f	Type of offspring^g	Offspring included^h	qPCR of the descendantsⁱ
1	10	15	51	3	Birth 3 asymptomatic	−	−	N	0	− \|−\| −
2	10	15	27	(5)	Deceased, autolysis of fetuses	+	+	F	0	N/A
3	10	15	51	0	Resorption	+	+	N/A	N/A	N/A
4	10	30	22	1	Abortion	−	+	F	1	+
5	10	30	42	3	Birth 1 asymptomatic, 2 dead	+	+	N	1	+\| +\|+
6	10	30	42	4	Birth 3 asymptomatic, 1 dead	+	+	N	1	+\| +\| +\|+
7	10	48	24	2	Birth 0 asymptomatic, 2 dead	+	+	N	1	+\|+
8	10	48	19	4	Birth 0 asymptomatic, 4 dead	−	+	N	1	+\| +\| +\|+
9	10	48	17	4	Birth 0 asymptomatic, 4 dead	−	+	N	1	+\| +\| +\|+
10	100	15	51	0	Resorption	+	+	N/A	N/A	N/A
11	100	15	51	0	Resorption	+	+	N/A	N/A	N/A
12	100	15	51	0	Resorption	+	+	N/A	N/A	N/A
13	100	30	36	4	Birth 4 asymptomatic, 0 dead	+	+	N	1	+\| +\| +\|+
14	100	30	36	2	Birth 0 asymptomatic, 2 dead	+	+	N	1	+\|+
15	100	30	40	3	Birth 3 asymptomatic, 0 dead	+	+	N	1	+\| +\|+
16	100	48	11	2	Abortion	−	+	F	1	+\|+
17	100	48	10	3	Abortion	−	+	F	1	+\| +\|+
18	100	48	20	4	Birth 4 living and malformed, 0 dead	+	+	N	1	+\| +\| +\|+
19	500	15	51	0	Resorption	+	+	N/A	N/A	N/A
20	500	15	14	0	Deceased, resorption	+	+	N/A	N/A	N/A
21	500	15	51	(1)	Birth 0 asymptomatic, 1 dead	+	+	N	0	N/A
22	500	30	37	(1)	Abortion, autolysis of fetus	+	+	F	0	N/A
23	500	30	17	0	Euthanasia due to 20% weight loss, fetal absorption	−	+	N/A	N/A	N/A
24	500	30	13	2	Euthanasia due to CNS-symptoms	−	+	F	1	+\| −^j
25	500	48	9	3	Deceased	−	+	F	0	− \|−\| −^k
26	500	48	9	4	Abortion	−	+	F	1	+\| +\| +\|+
27	500	48	12	1	Abortion	+	+	F	1	+
28	0	0	66	1	Birth 1 asymptomatic, 0 dead	−	−	N	1	−
29	0	0	66	3	Birth 3 asymptomatic, 0 dead	−	−	N	1	−
30	0	0	66	4	Birth 4 asymptomatic, 0 dead	−	−	N	1	−

^aNumber of sporulated oocysts, ^bassumed day of pregnancy, ^cduration from inoculation to birth, abortion or euthanasia. ^dIn the bracketed litters, all fetuses were highly autolytic and were therefore excluded from further analyses. ^e“ + ” represents a positive result, “-” a negative result, ^f“ + ” represents a positive result, “-” a negative result, ^g“N”: neonatal, “F”: fetal, ^h“1”: included, “0”: not included, ⁱ“ + ” represents a positive result, “-” a negative result for each individual offspring. ^jOnly the positive embryo was included in further analyses. ^kThe liver of the mother showed a positive result. These three fetuses were not included in the further examinations

ISSN: 1756-3305