Table 2 Primers and probes used to target Tibet Orbivirus genes
From: Isolation of Tibet Orbivirus from Culicoides jacobsoni (Diptera, Ceratopogonidae) in China
Target | Name of primer or probea | Sequences | Length of PCR products (bp) |
|---|---|---|---|
Seg4/VP4 | TIBOV-Seg4-F | 5-TGCGCTATACAGTGCAGAAG | 1145 |
TIBOV-Seg4-R | 5-AATCCGCCACATAAGATCC | ||
Seg5/NS1 | TIBOV-Seg5-F | 5-TTGCCACCAGATGCGTATCA | 707 |
TIBOV-Seg5-R | 5-GCTGTYGTAATCAAYGCTTCCA | ||
Seg9/VP6 | TIBOV-Seg9-F | 5-AAGAGCGGAAGGAAGAGAG | 855 |
TIBOV-Seg9-R | 5-GCTACGGTCAGGTCTACATC | ||
Seg9/VP6 | TIBOV-YG-S9-F | 5-CTACGGAACGAGGAGGGGAT | 99 |
TIBOV-YG-S9-R | 5-CTCGCTGCACATTTCCATCTC | ||
TIBOV-S9-Probe | 5-ATCAGCTCGTCCTCCTCCCTCTCGT |