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Table 2 Primers and probes used to target Tibet Orbivirus genes

From: Isolation of Tibet Orbivirus from Culicoides jacobsoni (Diptera, Ceratopogonidae) in China

Target Name of primer or probea Sequences Length of PCR products (bp)
Seg4/VP4 TIBOV-Seg4-F 5-TGCGCTATACAGTGCAGAAG 1145
TIBOV-Seg4-R 5-AATCCGCCACATAAGATCC
Seg5/NS1 TIBOV-Seg5-F 5-TTGCCACCAGATGCGTATCA 707
TIBOV-Seg5-R 5-GCTGTYGTAATCAAYGCTTCCA
Seg9/VP6 TIBOV-Seg9-F 5-AAGAGCGGAAGGAAGAGAG 855
TIBOV-Seg9-R 5-GCTACGGTCAGGTCTACATC
Seg9/VP6 TIBOV-YG-S9-F 5-CTACGGAACGAGGAGGGGAT 99
TIBOV-YG-S9-R 5-CTCGCTGCACATTTCCATCTC
TIBOV-S9-Probe 5-ATCAGCTCGTCCTCCTCCCTCTCGT
  1. TIBOV, Tibet Orbivirus
  2. a6-Carboxy-fluorescein (FAM) and quenching group BHQ1 were conjugated on the 5’ and 3’ ends of the probe, respectively