Table 3 Screening of Culicoides midges from Shizong County, Yunnan Province, China, for the presence of TIBOV by reverse transcription-quantitiative PCR
From: Isolation of Tibet Orbivirus from Culicoides jacobsoni (Diptera, Ceratopogonidae) in China
| Species | Midges tested by RT-qPCR (n) | Pools tested by RT-qPCR (n) | |||
|---|---|---|---|---|---|
| Total | Cq < 30 | 30 ≤ Cq < 35 | Cq > 35 | ||
| C. tainanus | 800 | 160 | 1 | 2 | 6 |
| C. jacobsoni | 400 | 80 | 1 | 0 | 2 |
| C. sumatrae | 300 | 60 | 0 | 0 | 0 |
| C. oxystoma | 300 | 60 | 0 | 0 | 0 |
| Culicoides (Trithecoides) spp.a | 120 | 24 | 0 | 0 | 1 |
| C. insignipennis | 80 | 16 | 0 | 1 | 0 |
| Total | 2000 | 400 | 2 | 3 | 9 |