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Table 3 Screening of Culicoides midges from Shizong County, Yunnan Province, China, for the presence of TIBOV by reverse transcription-quantitiative PCR

From: Isolation of Tibet Orbivirus from Culicoides jacobsoni (Diptera, Ceratopogonidae) in China

Species Midges tested by RT-qPCR (n) Pools tested by RT-qPCR (n)
Total Cq < 30 30 ≤ Cq < 35 Cq > 35
C. tainanus 800 160 1 2 6
C. jacobsoni 400 80 1 0 2
C. sumatrae 300 60 0 0 0
C. oxystoma 300 60 0 0 0
Culicoides (Trithecoides) spp.a 120 24 0 0 1
C. insignipennis 80 16 0 1 0
Total 2000 400 2 3 9
  1. Cq, PCR quantification cycle; RT-qPCR, reverse transcription-quantitiative PCR
  2. aCulicoides (Trithecoides) spp. refers to specimens belonging to this subgenus that have a yellow scutum