Table 1 DNA extraction efficiency on tenfold serial dilutions of Toxocara canis eggs (104 to 1) spiked in 10-g sand and 10 g-soil samples, by comparing two extraction kits, with or without an additional DNA purification and/or DNA dilution (1:10) step
From: Optimized DNA-based identification of Toxocara spp. eggs in soil and sand samples
Commercial kits | Sample type | Amount of spiked eggs | qPCR results quantification cycle (Cq) | |||
|---|---|---|---|---|---|---|
No clean-up step | AMPure® clean-up step | |||||
Undiluted DNA | Diluted DNA (1:10) | Undiluted DNA | Diluted DNA (1:10) | |||
DNeasy® Power Max® Soil Kit | Sand | 1 | Negative | Negative | Negative | Negative |
10 | 32.49 ± 0.82 | 35.40 ± 1.26 | 34.25 ± 1.51 | Negative | ||
100 | 29,39 ± 1,01 | 32.54 ± 1.68 | 32.58 ± 1.24 | 37.57 ± 0.24 | ||
1000 | 25,91 ± 0,61 | 29.11 ± 0.64 | 29.08 ± 1.02 | 34.04 ± 0.65 | ||
10,000 | 24.32 ± 0,53 | 26.61 ± 0.22 | 24.02 ± 0.14 | 27.45 ± 0.09 | ||
Soil | 1 | Negative | Negative | Negative | Negative | |
10 | Negative | Negative | Negative | Negative | ||
100 | Negative | Negative | Negative | 35.34 ± 1.26 | ||
1000 | Negative | Negative | Negative | 33.01 ± 0.17 | ||
10,000 | Negative | Negative | 29.23 ± 1.32 | 31.76 ± 0.73 | ||
FastDNA™ SPIN Kit for Soil | Sand | 1 | Negative | Negative | Negative | Negative |
10 | Negative | Negative | Negative | Negative | ||
100 | Negative | Negative | Negative | Negative | ||
1000 | Negative | Negative | Negative | Negative | ||
10,000 | 34.69 ± 0.23 | 37.66 ± 0.24 | 37.14 ± 1.11 | Negative | ||
Soil | 1 | Negative | Negative | Negative | Negative | |
10 | Negative | Negative | Negative | Negative | ||
100 | Negative | Negative | Negative | 37.17 ± 0.59 | ||
1000 | Negative | Negative | Negative | 35.77 ± 0.97 | ||
10,000 | Negative | Negative | Negative | 30.03 ± 1.00 | ||