Fig. 1

Domain organization, expression and immunization of PvPH and PvSOP26. a Schematics of PvPH and PvSOP26 showing domain organization and fragments expressed in yeast. Signal peptide, transmembrane regions, low complexity regions and the expressed segment are illustrated. b Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and western blot analysis of recombinant PvPH and PvSOP26. The left and middle panels show Coomassie-stained SDS-PAGE gels of the purified recombinant proteins. The right panel shows the western blot analysis using the mouse antisera against the respective recombinant proteins. M Protein marker in kiloDaltons. c Antibody titers against the recombinant proteins determined by enzyme-linked immunosorbent assay. Pooled serum samples were tested at twofold serial dilutions. Results are representative of three independent experiments. Bars (error bars) indicate the mean (±  standard error of the mean [SEM]) for three mice per group. **P < 0.01 (Student’s t-test).1 :32000 control vs PvPH: t₍₂₎ = − 7.64, P = 0.016; 1:32000 control vs PvSOP26: t₍₂₎ = − 10.733, P = 0.016