Quantitative PCR assay for the detection of Aedes vigilax in mosquito trap collections containing large numbers of morphologically similar species and phylogenetic analysis of specimens collected in Victoria, Australia

Table. 3 Genetic diversity indices and neutrality tests for all Ae. vigilax, using COI, alpha amylase and the zinc finger sequence

	Sample size	Number of haplotypes	Haplotype diversity	No. polymorphic sites	Nucleotide diversity estimate		Tajima’s D	Fu’s Fs
	Sample size	Number of haplotypes	Haplotype diversity	No. polymorphic sites	π	Θ	Tajima’s D	Fu’s Fs
COI
All	66	47	0.969	66	0.0144	0.0245	− 1.400	− 2.928*
Clade I	5	4	0.9	5	0.0037	0.0040	− 0.560	− 0.578
Clade II	16	11	0.974	16	0.0064	0.0087	− 1.141	− 1.114
Clade III	45	20	0.903	35	0.0054	0.0147	− 2.278*	− 3.871*
Victorian	12	12	1	21	0.0069	0.0117	− 1.814*	− 2.337*
Alpha amylase
All	66	64	0.9945	93	0.0126	0.0261	− 1.660	− 3.032*
Clade I	5	5	1	15	0.0089	0.0087	0.203	0.216
Clade II	16	16	1	43	0.0114	0.0167	− 1.344	− 1.675
Clade III	45	43	0.9989	76	0.0136	0.0261	− 1.395	− 2.668*
Victorian	12	11	0.9848	23	0.0080	0.0092	− 0.570	− 0.756
Zinc finger
All	66	57	0.98868	72	0.0086	0.01978	− 1.922*	− 3.328*
Clade I	5	3	0.7	5	0.0028	0.00305	− 0.562	− 0.578
Clade II	16	16	1	28	0.00782	0.01112	− 1.223	− 1.359
Clade III	45	41	0.99394	56	0.00871	0.01688	− 1.709	− 2.560*
Victorian	12	10	0.95455	14	0.0052	0.00632	− 0.757	− 1.063

ISSN: 1756-3305