Fig. 1

The multipronged approach designed to detect potential manipulation factors in the secretome of Schistocephalus solidus. On the left, the proteomic approach based on LC–MS/MS aims at describing the global proteomic composition of the proteome and the secretome of S. solidus. On the right, the genomic approach aims at producing a novel genome sequence and assembly of S. solidus to improve protein coding gene prediction and annotation for this parasite. The genome, whose quality is improved, can be used as a new reference database to infer proteins in the proteome and the secretome of S. solidus. Combined, these approaches allow us to characterize the secretome of S. solidus at the infective stage, including the uncovering of proteins detected only in that fraction and not in its proteome, thus representing potential manipulation factors