Fig. 3
From: Identification of anti-horn fly vaccine antigen candidates using a reverse vaccinology approach
SDS-PAGE and Western blotting analysis of the BI-HS009 antigen candidate produced in Pichia pastoris. a SDS-PAGE: 4.2 µg of BI-HS009 (009) were loaded and run in NuPAGE 4–12% Bis–Tris gels, followed by staining with Coomassie Brilliant Blue R-250. Arrows indicate stained protein bands that were excised and subjected to N-terminal sequencing. b Western blotting detection of BI-HS009 using the Western Breeze Chromogenic Western Blot Immunodetection Kit and anti-His tag antibodies. Stds = molecular weight standards (250 kDa – 10 kDa) (Precision Plus Protein All Blue) (Bio-Rad)