Identification of anti-horn fly vaccine antigen candidates using a reverse vaccinology approach

Parasites & Vectors

Table 2 Summary for the 10 selected vaccine antigen candidates

Antigen ID	Vacceed score (rank)^a	VaxiJen score (rank)^b	MS confirmed^c	Sequence verification^d		Expressed^e	Immunization trial
Antigen ID	Vacceed score (rank)^a	VaxiJen score (rank)^b	MS confirmed^c	Coverage (%)	Identity (%)	Expressed^e	Immunization trial
BI-HS001	0.999 (32)	1.5791 (16)	Yes	59	100	Small	No
BI-HS002	0.999 (32)	1.0257 (34)	No	46	99	Small	No
BI-HS003	0.944 (4701)	0.9715 (38)	No	48	97	Small	No
BI-HS004	0.937 (5322)	0.9323 (49)	Yes	–	–	None	No
BI-HS005	0.930 (5459)	0.9223 (51)	No	–	–	None	No
BI-HS006	0.998 (1055)	0.8890 (56)	Yes	54	99	Small + large	No
BI-HS007	0.937 (5322)	0.8353 (73)	No	43	100	Small	No
BI-HS008	0.943 (4931)	0.8248 (86)	Yes	–	–	None	No
BI-HS009	0.998 (1055)	0.8149 (88)	No	61	99	Small + large	Yes
BI-HS010	0.943 (4931)	0.8107 (90)	Yes	–	–	None	No

^aRank in Vacceed scores of all 36,282 transcripts analyzed (refer to Additional file 4 for detailed information)
^bRank in VaxiJen scores of all 535 transcripts analyzed (refer to Additional file 4 for detailed information)
^cDenotes whether antigen's amino acid sequence could be confirmed by mass spectrometry of proteins extracted from adult horn flies
^dVerifying transcript expression in adult flies: Coverage denotes average % of transcript that was covered by our RT-PCR and sequencing primer sets designed for analysis of wild fly RNA. Identity denotes the average % of nucleotide identity between the sequenced region of overlap between the transcript sequence and the RT-PCR product from wild fly RNA (refer to Additional file 7: Figure S1 for detailed information)
^eDenotes whether the antigen was successfully expressed in small-scale or large-scale P. pastoris expression and recombinant protein purification experiments conducted by Creative BioMart

ISSN: 1756-3305