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Table 3 Results of in vitro feeding assay with adult flies fed with blood from vaccinated animals (BI-HS009 or control) collected on days 21, 42, and 56

From: Identification of anti-horn fly vaccine antigen candidates using a reverse vaccinology approach

 

BI-HS009a

Controla

ANOVA

 

Total

Mean (SD)

Total

Mean (SD)

Fly mortality

 Day 21

6

1.50 (1.29)

1

0.25 (0.50)

F (1, 6) = 2.000, P = 0.207

 Day 42

4

1.00 (0.82)

5

1.25 (0.96)

 Day 56

5

1.25 (0.96)

3

0.75 (0.96)

Number of eggs laid

 Day 21

133

33.3 (22.2)

259

64.8 (20.6)

F (1, 6) = 4.447, P = 0.079

 Day 42

195

48.8 (21.4)

189

47.3 (27.2)

 Day 56

114

28.5 (18.5)

303

75.8 (26.7)

Number of pupae

 Day 21

106

26.5 (17.7)

217

54.3 (16.1)

F (1, 6) = 8.221, P = 0.028

 Day 42

155

38.8 (16.2)

163

40.8 (23.7)

 Day 56

95

23.8 (13.3)*

260

65.0 (16.7)

Number of newly emerged adults

 Day 21

83

20.8 (15.6)

198

49.5 (13.5)

F (1, 6) = 8.299, P = 0.028

 Day 42

83

20.8 (15.0)

119

29.8 (24.0)

 Day 56

92

23.0 (13.5)*

214

53.5 (15.3)

  1. SD standard deviation
  2. *Indicates significant difference at P < 0.05 between BI-HS009 and control group according to two-way repeated-measures ANOVA followed by the Šídák’s multiple comparisons test
  3. aFour cages with 20 flies each (10 males and 10 females) were used per group (BI-HS009 and control) for each blood collection date (days 21, 42, or 56)