Table 1 Information of primer sets used in this study
Gene | Sequence (5′-3′) | Size of target sequence | Amplified fragment |
|---|---|---|---|
ef1a-B-F | ACTAGTTCTAGAGCGGCCGCGTTGTCTTTTGGTATTAC | 958 | ef1a-B promoter sequence |
ef1a-B-R | CGCCCTTGCTCACCATTCTAGATTTCGTGGAGTTTTAACT | ||
gfp-bsd-F | ATGGTGAGCAAGGGCGAGGAG | 1113 | gfp-bad gene |
gfp-bsd-R | AGCTCCACCGCGGTGGCGGCCGCTTAGCCCTCCCACACATAAC | ||
rap-F | GGGAGGGCTAAGCGGCCGCGGCAGCTTCAAAGCG | 1362 | rap3' TR |
rap-R | GGGTCTTCTCCTTCGGCATTCTTAACACCAGGCAAT | ||
ef1a-orf-F | ATGCCGAAGGAGAAGACCC | 1347 | ef1α-B orf |
ef2a-orf-R | CCGCGGTGGCGGCCGCTCACTTCTTGGCGGCCTTCTGGGC | ||
efbs-F | GAGCGTCGAATTATTAACTGGG | 1924 | F1 |
efbs-R | CTTGTACAGCTCGTCCATGC | ||
gfor-F | GCCAAGCCTTTGTCTCAAGAAG | 3394 | F2 |
gfor-R | CTAGCAGTGTGTTAGCGTGC | ||
orf-R | GCACGATTCCATCGCTACAACG | 5960 | F3 (primer set efbs-F and orf-R) |