Table 2 Optimization of pyrosequencing assays using our own primers and the plasmid constructs previously described
% RT/STa type according to mixes | % of RT/ST observed by pyrosequencing (mean ± SD) | |||
|---|---|---|---|---|
Tr. trichiura | N. americanus | |||
Codon198 | Codon 200 | Codon 198 | Codon 200 | |
0 | 0 (± 0) | 0 (± 0) | 0 (± 0) | 8.8 (± 3.7) |
30 | 22.2 (± 1.9) | 21 (± 3.9) | 40.3 (± 6.6) | 38 (± 8.3) |
50 | 40.7 (± 2) | 38.7 (± 1.2) | 53.9 (± 2.6) | 52.2 (± 1.1) |
70 | 66.1 (± 2.2) | 64.5 (± 1.8) | 66.9 (± 4.1) | 67.5 (± 0.6) |
100 | 97.1 (± 2.2) | 97 (± 2.4) | 100 (± 0) | 92.8 (± 2.5) |