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Fig. 1 | Parasites & Vectors

Fig. 1

From: Three different mutations in the DNA topoisomerase 1B in Leishmania infantum contribute to resistance to antitumor drug topotecan

Fig. 1

Phenotypic characterization of L. infantum TPT-resistant mutants selected in vitro. a Concentration–response curves for L. infantum WT and TPT-resistant promastigotes in the presence of growing concentrations of TPT. EC50 values were calculated from concentration–response curves performed in triplicate after nonlinear fitting with GraphPad Prism 8.4.3 software: 25, 612, 519, and 602 µM for the WT, TPT700.1, TPT700.2, and TPT700.3, respectively. b Growth profiles of L. infantum WT and the three TPT-resistant mutants after reaching the last selection step. Parasites were seeded in M199 medium at a final concentration of 106 parasites ml−1, and their growth was monitored daily for 7 days by manual counting. For a and b, data are the mean ± SEM of three biological replicates. Statistical analyses were performed using unpaired two-tailed t-tests. c EC50 calculation for the three TPT-resistant mutants after 10, 30, and 60 passages without TPT in the cell culture media

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Parasites & Vectors

ISSN: 1756-3305

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