Table 1 Overview of the common mutated genes in the three TPT-resistant mutants
| Mutant | Gene ID | Mutation position | Allele frequency | Ref/Mut AA | Gene function |
|---|---|---|---|---|---|
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.01.0420 |
1073 1073 1073 |
Het Het Het |
A/E A/E A/E | Hypothetical protein conserved |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.09.0680 |
1388 1388 1388 |
Hom Hom Hom |
na na na | Hypothetical protein conserved |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.09.0950 |
17 17 17 |
Hom Hom Hom |
na na na | Polyubiquitin |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.12.0662 |
425 425 425 |
Het Het Het |
S/T S/T S/T | Surface antigen protein putative |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.19.1680 |
3792 3665 2940 |
Het Het Hom |
na V/A E/D | Hypothetical protein |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.19.1690 |
3956 3389 3956 |
Het Het Het |
S/N G/A S/N | Hypothetical protein |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.24.1120 |
771 776 771 |
Het Het Het |
S/R S/F S/R | Pre-mRNA splicing factor putative |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.27.0870 |
2572 2572 2572 |
Het Het Het |
R/G R/G R/G | Hypothetical protein conserved |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.28.2320 |
856 856 856 |
Hom Hom Hom |
N/Y N/Y N/Y | Hypothetical protein conserved |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.28.2390 |
1321 1321 1321 |
Hom Hom Hom |
na na na | Cyclin-dependent kinase-binding protein putative |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.28.2450 |
3758 3758 3758 |
Hom Hom Hom |
na na na | DNA topoisomerase ii |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.29.1450 |
242 242 242 |
Hom Het Hom |
A/V A/V A/V | Amastin-like protein |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.29.2240 |
1267 1267 1267 |
Hom Hom Hom |
L/F L/F L/F | Hypothetical protein conserved |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.31.1470 |
1088 1088 1109 |
Hom Hom Het |
na na na | Hypothetical protein unknown function |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.34.0710 |
2129 76 2843 |
Het Het Het |
G/D A/P D/G | Flagellar attachment zone protein putative |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.34.3220 |
560 572 724 |
Hom Het Hom |
F/Y G/A W/R | DNA topoisomerase IB large subunit |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.35.0490 |
16,451 1992 16,285 |
Hom Het Hom |
R/H na V/I | Proteophosphoglycan ppg4 |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.35.0510 |
7549 5278 2437 |
Het Het Het |
A/T V/L G/S | Proteophosphoglycan ppg4 |
|
TPT700.1 TPT700.2 TPT700.3 | LinJ.35.0550 |
1699 2471 2471 |
Het Het Hom |
A/T na na | Proteophosphoglycan ppg1 |