| |
WT
|
F187Y
|
G191A
|
W232R
|
|---|
|
Active site pentad and surrounding residues and DNA bases
|
|
ARG314:ASN452
| | |
69.42
| |
|
CYS329: ARG314
|
98.76
|
97.96
|
98.37
|
97.98
|
|
LYS352:ASP353
|
70.96
|
68.41
|
88.26
| |
|
ILE355:LYS352
|
57.14
|
73.93
|
96.42
|
75.04
|
|
ARG410:ALA406
|
44.32
|
61.96
|
97.86
| |
|
ARG410:ALA312
|
90.58
|
98.60
|
75.04
|
90.59
|
|
ARG410:TYR222s
|
59.80
|
83.62
| | |
|
ARG410:CYS451
| |
45.97
| | |
|
ALA414:ARG410
|
89.56
|
89.25
|
96.27
|
90.98
|
|
HIS453:ALA202s
|
79.59
|
85.58
|
90.06
|
86.04
|
|
SER214s:HIS453
|
85.85
|
93.02
|
76.47
|
88.41
|
|
TYR222s:SER218s
|
68.45
|
73.21
|
78.77
|
64.46
|
|
ARG314:THY +1
|
87.60
|
98.50
|
73.82
|
88.02
|
|
LYS352:THY −1
| |
52.21
|
68.58
|
53.88
|
|
LYS352:THY +1
| | | |
74.12
|
|
ARG410:THY +1
|
75.18
|
86.34
| | |
|
HIS453:THY +1
|
94.79
|
96.96
|
55.49
|
87.59
|
|
TYR222s:THY −1
|
65.22
|
80.60
| |
43.97
|
|
TYR222s:THY +1
|
55.33
| | |
70.77
|
|
Residues of point mutations and surrounding residues
|
|
PHE(Y)187:LYS198
|
99.25
|
99.14
|
99.33
|
99.48
|
|
GLY189:PHE(Y)187
| | | |
44.74
|
|
GLY197:PHE187
|
92.48
|
99.16
|
99.29
|
99.52
|
|
HIS193:GLY(A)191
| | |
46.89
|
61.69
|
|
TRP(R)242:LYS225
|
98.45
|
98.33
|
98.03
|
94.67
|
|
TRP(R)242:LYS182
| | | |
73.40
|
- Percentage of the hydrogen bonds established by the active site pentad with the surrounding residues (“s” following the residue number indicates that residue belongs to the small subunit)
