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Fig. 4 | Parasites & Vectors

Fig. 4

From: In vitro and in vivo efficacy of thiacloprid against Echinococcus multilocularis

Fig. 4

Thiacloprid against E. multilocularis protoscoleces and inhibited acetylcholinesterase activity. a Survival of protoscoleces after treatment with thiacloprid. The protoscoleces were exposed to thiacloprid for 7 days. To evaluate the survival of protoscoleces, a 0.1% eosin staining exclusion method was used daily. The positive control was 5 μM PZQ. The data are presented as mean ± SD obtained from three independent experiments. b Morphologies of protoscoleces after treatment with thiacloprid. After treatment with 5 μM thiacloprid for 4 days, eosin staining was used to observe the protoscoleces. The red arrow shows the dead protoscoleces and the black arrow shows the live protoscoleces. Scale bar = 200 μm. c SEM of E. multilocularis protoscoleces incubated in vitro with 5 μM thiacloprid for 4 days. d Thiacloprid inhibited acetylcholinesterase activity. BW284c51 was used as the positive control. The data are presented as mean ± SD obtained from five independent experiments. e Thiacloprid inhibits glucose consumption. The protoscoleces or germinal cells were treated with 5 μM thiacloprid, and relative glucose utilization was determined. The data are presented as mean ± SD obtained from three independent experiments. *P < 0.05 and **P < 0.01

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