Fig. 5

Amplification plots of the vgsc gene fragment of studied tropical bed bugs using TaqMan real-time PCR. a Amplification of the mutant (with M918I mutation) and wild-type DNA fragments in both samples. b Amplification of mutant and wild-type fragments in all samples. The solid blue and green lines marked with 0.074577 and 0.2 represent the threshold cycle (Ct) in the mutant and wild groups, respectively. The ΔRn in the Y axis is the difference of fluorescence between the sample and baseline. The baseline of mutant and wild groups (represented as blue and green lines, respectively) is the line at which there are no background fluorescence peaks crossing over it