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Table 4 Genetic diversity indices and neutrality tests (Fu’s Fs and Tajima’s D) of the ITS2 region in 17 Hyrcanus group members

From: Molecular phylogeny of the Anopheles hyrcanus group (Diptera: Culicidae) based on rDNA–ITS2 and mtDNA–COII

Species

N

S

Pi

H

Hd

Fu's Fs

Tajima's D

An. paraliae

26

1

An. pursati

22

1

An. belenrae

26

1

0.000200

2

0.077

− 1.094

− 1.15559

An. peditaeniatus

117

58

0.004080

11

0.18

− 2.072

− 2.67451***

An. crawfordi

53

12

0.011000

4

0.491

9.501

2.50567

An. hyrcanus

115

11

0.001670

9

0.509

− 4.462**

− 1.78638**

An. hyrcanus spIR

7

6

0.00597

3

0.524

1.934

0.45159

An. pullus

33

2

0.000900

3

0.225

− 0.357

− 0.41482

An. kleini

13

1

An. lesteri

24

54

0.018260

10

0.62

1.406

− 1.88497**

An. sineroides

7

4

0.002710

4

0.714

− 1.217

− 1.43414

An. liangshanensis

4

1

0.001120

2

0.5

0.172***

− 0.61237

An. pseudopictus

30

1

An. nitidus

46

4

0.002250

4

0.648

1.009

0.42695

An. argyropus

26

4

0.004320

4

0.618

2.283

2.52775

An. nigerrimus

41

19

0.003140

4

0.45

2.075

− 2.105**

An. sinensis

231

17

0.003240

23

0.623

− 16.474***

− 1.60196*

  1. Species represented by < 3 specimens were excluded from the analyses.
  2. *, **, ***Significance of Fu’s Fs and Tajima’s D values at *P < 0.05, **P < 0.02 and ***P < 0.001
  3. N, number of sequences; S, number of polymorphic sites; Pi, nucleotide diversity; H, number of haplotypes; Hd, haplotype diversity