Molecular phylogeny of the Anopheles hyrcanus group (Diptera: Culicidae) based on rDNA–ITS2 and mtDNA–COII

Parasites & Vectors

Table 5 Genetic diversity indices and neutrality tests (Fu’s Fs and Tajima’s D) of the COII gene in 13 Hyrcanus group members

Species	N	S	Pi	H	Hd	Fu's Fs	Tajima's D
An. paraliae	21	5	0.00127	4	0.533	− 0.286	− 1.1345
An. pursati	11	2	0.00149	2	0.509	2.343	1.50194
An. belenrae	3	6	0.00584	3	1	0.134***	–
An. peditaeniatus	83	21	0.00322	17	0.815	− 7.932*	− 1.64465*
An. hyrcanus	35	45	0.01102	24	0.963	− 9.744**	− 1.31777^#
An. pullus	6	6	0.00351	5	0.933	− 1.728	− 0.49605
An. kleini	16	20	0.00765	15	0.992	− 9.839**	− 0.84867
An. crawfordi	48	32	0.01177	21	0.863	− 2.955	0.20133
An. lesteri	45	73	0.01529	38	0.986	− 24.744***	− 1.68623*
An. nitidus	24	19	0.00602	21	0.986	− 18.427***	− 0.9986
An. argyropus	17	12	0.00561	10	0.919	− 2.458	0.00026
An. nigerrimus	27	33	0.00449	16	0.906	− 8.892**	− 2.38098**
An. sinensis	163	40	0.00436	52	0.866	− 55.03***	− 1.97306**

Species represented by < 3 specimens were excluded from the analyses.
#, *, **, ***Significance of Fu’s Fs and Tajima’s D values at #P < 0.10, *P < 0.05, **P < 0.02 and ***P < 0.001
N, number of sequences; S, number of polymorphic sites; Pi, nucleotide diversity; H, number of haplotypes; Hd, haplotype diversity

ISSN: 1756-3305