Fig. 4
Inhibition of miR-497 in mice reduced carbon tetrachloride (CCl4)-induced liver damage. A mouse model of liver fibrosis was established by injection of CCl4 for 6 weeks, and the mice were treated with highly hepatotropic rAAV8-anti-miR-497 (anti-miR-497) or control rAAV8-scramble vectors (anti-SCR) with a single dose of 1 × 1012 virus genomes or PBS by tail vein injection onset of CCl4 injection. a The relative expression of miR-497 in the livers of mice from different groups. b The relative expression of Smad7 in the livers of mice from different groups. c Histological analysis of liver injuries by H&E staining in indicated groups. d The activities of ALT in the sera from each mouse were determined using commercial kits. *P < 0.05, **P < 0.01, *** P < 0.001, compared with the indicated group. All data represent at least three independent experiments