Fig. 1

Entomological bioassay. a Ten specimens of third-, fourth- and fifth-instar nymphs of Triatoma brasiliensis were placed in a glass pot with the top covered with a fine mesh and allowed to blood-feed directly on the abdomen of fluralaner (Bravecto^®)-treated dogs and on control animals for 30–40 min. b, c Blood-feeding by insects on a dog from the treated group. dTriatoma brasiliensis after finishing a blood meal. The insects were subsequently maintained in an incubator under standard conditions (28 °C; 50% relative humidity) and monitored at 24, 48, 72, 96 and 120 h after blood meal to determine the mortality rate