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Table 2 Details of the mosquito specimens used for phylogenetic analysis in this study

From: Updated distribution of anopheline mosquitoes in Hokkaido, Japan, and the first evidence of Anopheles belenrae in Japan

Species Specimen code Years collected Collection site GenBank Accession No. Reference
Country Prefecture /province Area GPS coordinates Habitat (method)
Latitude (N) Longitude (E) Adulta Larva
An. anthropophagus SMMU-FK1   China Faku       AY803792 [33]
An. belenrae Akan44 2004 Japan Hokkaido Tsurui 43.1129 144.3286 Open grassland to coniferous forest (DT)   LC634739  
Kushiro10 2005 Japan Hokkaido Kushiro 43.0668 144.2976   Lake LC634740  
Kushiro201 2008 Japan Hokkaido Kushiro 43.0668 144.2976 Lakeside (SW)   LC634741  
Kushiro313 2009 Japan Hokkaido Kushiro 43.0668 144.2976 Lakeside (SW) Lake LC634742  
Kushiro418 2010 Japan Hokkaido Kushiro 43.0668 144.2976   Pond LC634743  
Kawakami60 2010 Japan Hokkaido Shibecha 43.1531 144.5072   Swamp LC634744  
Akan712 2011 Japan Hokkaido Tsurui 43.1129 144.3286   River stagnant LC634745  
Kushiro503 2011 Japan Hokkaido Kushiro 43.0756 144.2778 Cowshed (AS)   LC634746  
Nakagawa807 2011 Japan Hokkaido Toyokoro 42.8206 143.5394 Cowshed (AS)   LC634747  
Nakagawa26 2014 Japan Hokkaido Toyokoro 42.8183 143.5417   Swamp LC634748  
Isolate 3   South Korea       AY375466 [34]
An. engarensis Abashiri02 2001 Japan Hokkaido Ozora 43.8748 144.1291 Cowshed (SW)   AB159604  
Abashiri18 2002 Japan Hokkaido Ozora 43.8647 144.1082 Cowshed (SW)   LC634749  
Yubari25 2003 Japan Hokkaido Kuriyama 42.9921 141.8794 Cowshed (SW)   LC634750  
Yufutsu20 2003 Japan Hokkaido Mukawa 42.5993 141.9595 Cowshed (SW)   LC634751  
Kameda40 2003 Japan Hokkaido Nanae 41.9792 140.6951 Cowshed (AS)   LC634752  
Daisen55 2005 Japan Akita Daisen 39.5 140.425 Cowshed (AS)   LC634753  
Yufutsu115 2014 Japan Hokkaido Mukawa 42.5894 141.9444 Cowshed (AS)   LC634754  
L1532 2014 Japan Hokkaido Naganuma 43.0685 141.7303   Paddy field LC634755  
A14-22 2014 Japan Hokkaido Atsuma 42.7 141.8334 Shrub (DT)   LC634756  
L1468 2014 Japan Hokkaido Tomakomai 42.6401 141.7883   Swamp LC634757  
An. kleini    South Korea       DQ177501b  
An. lesteri Abashiri01 2001 Japan Hokkaido Ozora 43.8748 144.1291 Cowshed (SW)   AB159606  
Abashiri17 2002 Japan Hokkaido Ozora 43.8748 144.1291 Cowshed (SW)   LC634758  
Abashiri42 2003 Japan Hokkaido Ozora 43.8647 144.1082 Cowshed (SW)   LC634759  
Hakodate31 2003 Japan Hokkaido Hakodate 41.7706 140.8619 Cowshed (AS)   LC634760  
Kamiiso35 2003 Japan Hokkaido Shiriuchi 41.6243 140.428 Cowshed (AS)   LC634761  
Kameda38 2003 Japan Hokkaido Nanae 41.9792 140.6951 Cowshed (AS)   LC634762  
Kushiro317 2009 Japan Hokkaido Kushiro 43.0668 144.2976   Lake LC634763  
Kushiro501 2011 Japan Hokkaido Kushiro 43.0756 144.2778 Cowshed (AS)   LC634764  
A2651 2015 Japan Hokkaido Atsuma 42.6407 141.7871   Pond LC634765  
Specimen 1 (type B) South Korea       AJ620899 [25]
Specimen 2 (type C) South Korea       AJ620900 [25]
An. pullus   China Henan       AY186792 [22]
An. sinensis Yokohama08 2001 Japan Kanagawa Yokohama 35.5551 139.4882 Resident (SW)   AB159603  
Misawa391 2007 Japan Aomori Misawa 40.8379 141.3721 Lakeside (DT)   LC634766  
GLVN59 2007 Vietnam Gia Lai Chu Se 13.6558 108.0779 Cowshed (SW)   LC634767  
Echizen379 2009 Japan Fukui Echizen 35.8903 136.1961 Cowshed (DT)   LC634768  
Kaifu353 2009 Japan Tokushima Kaiyo 34.0072 134.6373   Pond LC634769  
Isolate 1   South Korea       AY375464 [34]
An. sineroides Fukagawa13 2002 Japan Hokkaido Fukagawa 43.7499 142.0756 Cowshed (SW)   AB159605  
Toyama80 2007 Japan Toyama Toyama 36.6029 137.2682 Cowshed (DT)   LC634770  
Kushiro343 2009 Japan Hokkaido Onbetsu 42.9644 143.8848   River LC634771  
Hida386 2009 Japan Gifu Hida 36.3947 137.3745 Cowshed (DT)   LC634772  
SINEK02   South Korea       GU384724 [35]
  1. aAdult mosquitoes were collected in cowsheds and around their habitats using dry-ice traps (DT), aspirators (AS), and sweeping nets (SW)
  2. bITS2 sequence was directly submitted to the GenBank database