Fig. 4

Evidence of miR-155-5p being enriched in Tg-DC-Exo and delivered to recipient cells via exosomes. a qRT-PCR confirmation of the abundance of miR-155-5p in Tg-DC-Exo and DC-Exo. b After RAW264.7 cells were treated with 120 µg/ml Tg-DC-Exo or DC-Exo for 24 h, the miR-155-5p transcription levels were quantified with qRT-PCR and represented as the fold change relative to the U6 level (set to 1); normal cells were set as the negative control. c DC2.4 cells were pre-cultured in upper transwell chambers overnight. As indicated, the DC2.4 cells were pretreated with GW4869 or not treated, followed by infection with RH tachyzoites for 30 min or non-infection, and then the unrecruited tachyzoites were washed off. Synchronously, RAW264.7 cells were cultured in the lower chambers together with the infected DC2.4 cells in the upper chambers, in the same well for 28 h. The miR-155-5p transcription levels in each lower chamber were detected with qRT-PCR, and represented as the fold change relative to the U6 level (set to 1); normal cells were set as the negative control. Data are presented as mean ± standard deviation (SD). Student’s t-test and one-way ANOVA were used for the significance analysis, and Tukey’s multiple-group test was used for multiple-group comparisons. Each experiment was carried out three times for statistical analysis. (** P < 0.01 and ***P < 0.001)