Fig. 5

Tg-DC-Exo-enriched miR-155-5p promotes macrophage M1 polarization. After the indicated treatment, the levels of mRNAs in RAW264.7 cells were quantified with qRT-PCR and are represented as the fold change relative to U6 (set to 1) for miR-155-5p or relative to GAPDH (set to 1) for IL-6, iNOS, and TNF-α. The relative transcription levels of IL-6, iNOS, and TNF-α in the miR-155-5p mimic-transfected RAW264.7 cells at 18 h post-transfection (a) and in the miR-155-5p inhibitor-transfected cells at 24 h post-transfection (b). Western blot assay for iNOS expression in the miR-155-5p mimic transfection group and the other groups as control at 48 h post-transfection (c, left). Densitometric quantitation of each band in c-left was applied using ImageJ software (c, right). The mimics control transfection, and mock cells were set as negative controls, and the lipopolysaccharide (LPS) treatment was set as the positive control. SPSS software was used for statistical analysis. One-way ANOVA was used for between-group comparisons, and Tukey’s multiple-group test was used for multiple-group comparisons. Each experiment was carried out three times (*P < 0.05, **P < 0.01 and ***P < 0.001)