Fig. 7

Tg-DC-Exo miR-155-5p targeting socs1 to promote the inhibitory effect of interferon-γ (IFN-γ) on T. gondii proliferation through the NF-κB pathway. Detection of socs1 transcription with qRT-PCR at 24 h post-transfection and translation with western blot at 48 h post-transfection in RAW264.7 cells. a, b Transfection with miR-155-5p mimics or normal control miRNA (mimic-NC). c, d Transfection with the siRNA targeting socs1 (si-socs1) and the normal control siRNA (si-NC). e, f Detection of the phosphorylation levels of p65 and IKBα in the NF-κB pathway in the RAW264.7 cells transfected with miR-155-5p mimics, normal control siRNA (mir-NC), si-socs1, or si-NC for 36 h. g Detection of the T. gondii B1 gene copies in the RAW264.7 cells transfected with si-socs1 or si-NC for 24 h, and stimulated with IFN-γ for 24 h, then infected with T. gondii for 24 h. Densitometric quantitation of each band in b, d, and e was applied using ImageJ software. One-way ANOVA was used for between-group comparisons, and Tukey’s multiple-group test was used for multiple-group comparisons. Each experiment was carried out three times (**P < 0.01, ***P < 0.001)