Fig. 1

Vector competence to ZIKV in two Aedes aegypti strains. a Infection rate (IR), dissemination rate (DR), transmission rate (TR) and transmission efficiency (TE) of two Ae. aegypti strains to ZIKV. A total of 30 mosquitoes were analyzed per time point, except for 20 dpi (20 individuals). b Viral RNA copies in the midgut, salivary gland and saliva of the JG and MD Ae. aegypti strains at different time points after ZIKV infection. The line represents the median of results at each time point. c Normal C6/36 cells (i) or C6/36 cells inoculated for 7 days with saliva from JG (ii) or MD (iii) mosquito strains infected with ZIKV (×100 magnification). The results shown are combined results from three independent experiments. The IR, DR, TR and TE of the two Ae. aegypti strains were compared using Fisher’s exact test. Viral titers were compared using the Mann–Whitney U-test to determine differences between the two strains at different times. Abbreviations: JG, Jiegao strain; MD, Mengding strain