Fig. 8

Activity of rHc-CBP-1 on bovine peripheral blood mononuclear cells. Relative transcript abundance of pro-inflammatory cytokines (TNFα (a), IL-1 (b), IL-6 (c)) and co-stimulatory markers (CD40 (d), CD80 (e) and CD86 (f)) were evaluated on bovine PBMCs following 72 h of incubation with 0.1, 1 and 10 μg/ml concentrations of rHc-CBP-1. Data are reported as the mean (M) of two biological and three technical replicates. Statistically significant differences between media controls vs samples, inhibitor vs mix of inhibitor and rHc-CBP-1 (0.1, 1 and 10 µg/ml) and rHc-CBP-1 (0.1, 1 and 10 µg/ml) vs mix of inhibitor and rHc-CBP-1 (0.1, 1 and 10 µg/ml) are defined as follows: (*), ( +), (#) = p ≤ 0.05; (**), (+ +), (##) = p ≤ 0.01; (***), (+ + +), (###) = p ≤ 0.001. The arrows indicate the direction of change in transcript abundance normalized to media only