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Table 2 Overview of primers and probes used for the in-house qPCR-screenings of three pathogenic agents

From: A community approach of pathogens and their arthropod vectors (ticks and fleas) in dogs of African Sub-Sahara

Target

In-house forward primer

In-house reverse primer

In-house hydrolysis probe

Hepatozoon canis

GGCAGTGACGGTTAACGGGGG

GCACCAGACTTGCCCTCCAATTG

CCGGAGAGGGAGCCTGAGAAACGG

Dirofilaria immitis

  

CTTTGGAATATGTGTTTTTTTGGAGAGCCCTC

Babesia felis

AAGAAGCTCGTAGTTGAATTTCTGCC

GAGAAGCCGAAGCAACACAAATCCAG

TGCGTTTTCCGACTGGCTTGGCA

  1. For all PCRs, the final forward and an reverse primer concentrations were 400 nM. The final probe concentration was 200 nM
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Parasites & Vectors

ISSN: 1756-3305

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