Fig. 4

Reduction of AF for immunofluorescence assay of calreticulin (CRT) in female and male schistosomes. a Immunofluorescence staining for SjCRT in female. Scale-bar: 100 μm. b Immunofluorescence staining for SjCRT in male. Scale-bar: 500 μm. c Merged immunofluorescence images of different body regions of female and male worms; i the oral sucker and oesophagus of female, scale-bar: 10 μm; ii the tegument of female, scale-bar: 10 μm; iii the ventral sucker of male, scale-bar: 50 μm; iv the dorsal tegument of male worms, scale-bar: 50 μm. ‘Merge’ represented the merged images of DAPI/EGFP/SjCRT image. DAPI column is a staining for the nuclei (blue). EGFP column is not any staining and just as a control for detecting AF. SjCRT column is an immunostaining for SjCRT (red). Abbreviations: aeo, the anterior end of oesophagus; t, tegument; vs, ventral sucker