Table 1 Fluorescence image acquisition using CLSM
Acquisition channel | Fluorophore used | Excitation (nm) | Emission (nm) | Image display color |
|---|---|---|---|---|
DAPI | DAPI | 405 | 450 | Blue |
EGFP | NA | 488 | 515 | Green |
Cy3 | Cy3 | 532 | 590 | Red |
mCherry | NA | 561 | 610 | Red |
AF647 | NA | 640 | 665 | Purple |
Acquisition channel | Fluorophore used | Excitation (nm) | Emission (nm) | Image display color |
|---|---|---|---|---|
DAPI | DAPI | 405 | 450 | Blue |
EGFP | NA | 488 | 515 | Green |
Cy3 | Cy3 | 532 | 590 | Red |
mCherry | NA | 561 | 610 | Red |
AF647 | NA | 640 | 665 | Purple |