Table 1 Composition [mM], osmolarity and pH of buffer solutions for Ussing chamber experiments
From: Changes in porcine nutrient transport physiology in response to Ascaris suum infection
Buffer I (serosal) | Buffer II (mucosal) | Buffer III (mucosal) | |
|---|---|---|---|
NaCl | 113.6 | 113.6 | 113.60 |
KCl | 5.4 | 5.4 | 5.40 |
HCl (1n) | 0.2 | 0.2 | 0.20 |
MgCl2·6H2O | 1.2 | 1.2 | 1.20 |
CaCl2·2H2O | 1.2 | 1.2 | 1.20 |
NaHCO3 | 21.0 | 21.0 | 2.00 |
Na2HPO4·2H2O | 1.5 | 1.5 | 0.37 |
Glucose | 5.0 | – | – |
Mannitol | 1.2 | 1.2 | 32.94 |
HEPES | 7.0 | 20.0 | – |
Na-Gluconate | 6.0 | – | 19.83 |
1n NaOH | – | 6.0 | – |
NaH2PO4·H2O | – | – | 1.13 |
Osmolarity | 290 mosmol/l | 292 mosmol/l | 300 mosmol/l |
pH | 7.4 | 7.4 | 6.4 |