Table 2 Primer pairs and TaqMan™ MGB probes for quantitative real-time PCR
From: Changes in porcine nutrient transport physiology in response to Ascaris suum infection
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) | TaqMan™ MGB probe (5′–3′) | Amplicon length (bp) |
|---|---|---|---|---|
TBP | CTGCCCGGTTATTTATATTTAGA | AGTCCAATCAATTGTTGAGG | VIC-ACTTACTGCTGTTGAC-NFQ | 118 |
PPIA | GCAGACAAAGTTCCAAAGA | CACCCTGGCACATAAATC | 6-FAM-AACTTCCGTGCTCT-NFQ | 106 |
GLUT1 | ATCCCATGGTTCATTGTG | CACAGTTGCTCCACATAC | VIC-AACTCTTCAGCCAG-NFQ | 131 |
GLUT2 | GGAAGAAGCATCAAGTGAA | GATCCCATTGATTCCAGAAA | 6-FAM-CATCAGTGCTACTAGA-NFQ | 127 |
SGLT1 | CACTCAGTCGGATGTCTA | CCACAACTCTTAAAATAACATTCA | VIC-CACTGACATGCTGA-NFQ | 133 |
IL-4 | CTTCGGCACATCTACAGA | TCGTCTTTAGCCTTTCCA | 6-FAM-CTCTTCTTGGCTTCA-NFQ | 148 |
IL-13 | CTCTGGTTGACTCTGGTC | TCTGGTTCTGGGTGATATTG | 6-FAM-TTGCTCTCACCTGCTT-NFQ | 127 |
HIF1α | CTGGACACAGTGTGTTTG | GCTAGTTAAGGTACACTTCATTC | VIC-TACTCATCCGTGCGAC-NFQ | 149 |
Stat6 | CTCAGATGCCTTCTGCTG | GTCCCTCTGATATATGCTCTC | 6-FAM-TGCTATCTGCCACT-NFQ | 142 |
PepT1 | TCGGCTGGAATGACAATC | GGTGTAGACGATGGACAAC | VIC-TCCACTGCCATCTA-NFQ | 143 |