Fig. 8

The possible anti-tumor and anti-infection immunity stimulated by RH-Δompdc. RH-Δompdc mutant was constructed with the CRISPR/Cas9 technology. RH-Δompdc could multiply normally in the medium supplemented with uracil, but it was cleared without uracil and in vivo. RH-Δompdc could induce host immune responses, both in vitro and in vivo, by promoting the expression of inflammatory cytokines, thus protecting the mice from reinfection by the RH strain. The RH-Δompdc tachyzoites in situ inoculation to 4T1 tumors inhibited the tumor growth, promoted the survival and reduced the lung metastasis of the tumor-bearing mice. After the RH-Δompdc treatment, the secretion of Th1 cytokines such as IL-12 and IFN-γ were significantly elevated in both the serum and TME, so the immune cells may be activated to recognize and kill tumor cells. Our study indicated a highly potential biotherapy for RH-Δompdc being used in cancer treatment