Table 1 Primers used for PCRs and vector constructions
From: MiR-279-3p regulates deltamethrin resistance through CYP325BB1 in Culex pipiens pallens
Name of primer | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|
miR-279-3p (qRT-PCR) | ACACTCCAGCTGGGTGACTAGATCCACAC | TGGTGTCGTGGAGTCG |
U6 | GCTTCGGCTGGACATATACTAAAAT | GAACGCTTCACGATTTTGCG |
CYP325BB1 | TGCTGACCAGCGAACGAA | AGACCACCTTTCACCATCCC |
β-actin | AGCGTGAACTGACGGCTCTTG | ACTCGTCGTACTCCTGCTTGG |
CYP325BB1 3′-UTR-WT | TATCGGCTGTGGACTGACCTT | AAACCCATTTGGCATAAGACG |
CYP325BB1 3′-UTR-Δ | TATCGGCTGTGGACTGACCTT | CCAAGCTTTTTTTGCCCACCAAGGTTT |
pIB/V5-His-CYP323BB1 | GGACTAGTGAGATGGAAATGCTGTTCGAAGTGCTCC | CCGCTCGAGCGTTTATTTCTCTTAGTCAACCAAACT |