Fig. 3

Hc-DAF-5 is involved in differentiation from xL3 to L4 and interacts with Hc-DAF-3 in H. contortus. a, b The effects of RNAi on the development of H. contortus xL3 in vitro. a The transcriptional changes of Hc-daf-5 in H. contortus after soaking for 72 h, detected by real-time PCR. b L3 developmental rate in vitro for 5 days following RNAi. *P < 0.05; **P < 0.01; ***P < 0.001. Graphs in panels a and b show mean ± SEM from three independent experiments. c Assessment of the interaction between Hc-DAF-3 and Hc-DAF-5 in live mammalian cells using BiFC. BHK21 cells are co-transfected with plasmids, and images are acquired at 20 h after transfection. Scale bars: 100 μm. Full-length Hc-DAF-3 interacts with the SDS box of Hc-DAF-5 (HcDAF3-HA-KN151 and HcDAF5-SDS-Myc-LC151), while the MH2 domain of Hc-DAF-3 also interacts with the SDS box of Hc-DAF-5 (HcDAF3-MH2-HA-KN151 and HcDAF-5-SDS-Myc-LC151). Positive controls (bJun-HA-KN151 and bFos-Myc-LC151) and negative controls (HA-KN151 and HcDAF5-SDSMyc-LC151) are also shown