Fig. 2

Genetic diversity of fragment of 16S rRNA gene for the detection of Asaia sp. in An. coluzzii, An. gambiae and An. funestus at Mibellon, Tibati, Gounougou, Obout, Elende, Elon and Bankeng. a Phylogenetic tree of the sequences based on the 16S rRNA gene. Asaia sequences of the Anopheles populations were used to construct the phylogenetic tree based on the maximum likelihood method with 1000 bootstrap replicates. The sequences of Asaia were aligned against close reference sequences. The bootstrap values under 50% were discarded. b Nucleotide sequence variant network. Nucleotide sequence variant networks were constructed using Asaia sequences in the TCS program. H1–H7 represent the different nucleotide sequence variants, and each color represents each mosquito species according to locality. c Alignment of nucleotide sequence variant showing the polymorphic sites