Fig. 1

The cloning of the ecdysteroid receptor heterodimeric complex (EcR and USP) of Rhipicephalus haemaphysaloides. The ORFs of RhEcR1, RhEcR2, RhEcR3, RhUSP1, and RhUSP3 from R. haemaphysaloides were cloned, sequenced, and characterized in silico, and designated (a, b, c, and d). Nucleotide and deduced amino acid sequences of RhEcR1, RhEcR2, RhEcR3, RhUSP1, and RhUSP3 from R. haemaphysaloides (a and b). Putative DNA-binding domain (DBD) is boxed and ligand-binding domain (LBD) is underlined. Two C4-type zinc-finger motifs (ZF_C4) have DNA-binding regions of some well-characterized families of nuclear receptors, and each ZF_C4 is characterized by four cysteine residues, which are marked with arrows, that coordinate zinc and do not share sequence similarity. The distances between the cysteine residues are not conserved. The domain structure is drawn for RhEcR1, 2, and 3 and RhUSP1 and 3 (c and d)